01941nas a2200157   4500008004100000245007800041210006900119300001100188490000600199520145200205100001901657700002001676700001801696700002001714856004901734       2020                        eng d00aIn-Silico Analysis of Silk Serpin-2 Protein from Arachnocampa richardsae.0 aInSilico Analysis of Silk Serpin2 Protein from Arachnocampa rich  a98-1060 v73 a<p>Silk Serpin-2 protein originates in Arachnocampa richardsae species which exhibits the presence of unexplored nucleotide sequence. Whole-genome annotations for <em>Arachnocampa richardsae</em> have not been accomplished and there are no known functions of Silk Serpin-2 until now. Therefore in this paper, we studied specifications like phylogenetic analysis, conservative residue, and conserved domain, secondary and 3D structure, physicochemical properties, and functional importance of Silk Serpin-2. The methodology used for the study involve software tools like MEGA X, Bio Edit, PSIPRED and Swissprot for the overall insilico study of the Silk Serpin-2 protein. Reliable and effective methods for 3D structure modeling were used and validated using the Ramachandran Plot. Results showed that the presence of silk Serpin-2 in the cell is responsible for many processes like regulation of metabolic process (88.08%), catabolic processes (69%), and catalytic activity (85.7%). The tools were used to discover the physicochemical properties from number of amino acids to the instability index. The secondary structure and 3D model of the protein were predicted which represented valid amino acid percentage in the allowed region of Ramachandran plot. The study may prove to be a reference for revealing further applications of the protein Silk Serpin-2 and the implementation of this technique will aid to expose other unknown proteins.</p>
1 aChougale, Amit1 aMantri, Shivani1 aKagale, Sneha1 aVedante, Shruti  uhttps://sciaroidea.myspecies.info/node/60134